A NEW CULTURE-MEDIUM FOR THE GONOCOCCUS

Trypsin digesting medium added with horse plasma for the culture of the gonococcus.

To identify Neisseria-like colonies suspected to be gonococci, it is necessary to use the " oxydase" reaction of Gordon and McLeod (1928); colonies sprayed with a 1 per cent. solution of dimethyl paraphenylene hydrochloride turn pink immediately on contact with the oxidizing fluid, and then black in 2 to 3 minutes. If blood media are used, the " oxydase" reaction is not readily visible owing to...

P REPARATION OF A NEW MEDIUM FOR IN VITRO CULTURE OF BORRELIA MICROTTI AND BORRELIA PERSICA

Borrelia microtti and Borrelia persica are two Iranian strains of spirochetes whose vectors are Ornithodorous tholozani and Ornithodorous erraticus which are responsible for relapsing fever. BSK medium has been used for in vitro culture of other strains of borrelia, although the two mentioned strains could not be successfully cultured in it. We have modified BSK medium by using guinea pig ...

Human Placenta as an Enriching Medium for the Gonococcus

New blood culture medium.

A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involved the introduction of blood (and bacteria) into the growth medium with the dye...

Transportation of material for the culture of the gonococcus.

Until the beginning of World War lI the culture method was not extensively used in the diagnosis of gonorrhoea; diagnosis by culture was made only where laboratory facilities were available close to the clinics. The rapidly increasing incidence of gonorrhoea-especially among soldiers-made it desirable to obtain better diagnosis and control of the disease, and it became necessary to provide clin...